EF24 reduces hemorrhage-induced expression of TLR4. (A) Immunohistochemical analysis of TLR4 expression in liver tissues of rats subjected to sham surgery (Ctrl), hemorrhage (HS) and hemorrhage with EF24 administration (HS → EF24). The light blue stain represents the degree of expression of TLR4. (B) Quantitation of IHC values (* p < 0.05 vs. Ctrl and # p < 0.05 vs. HS; n = 3/group). (C) Immunoblots showing the TLR4 protein expression in three different sets. To demonstrate equal loading, the membranes were stripped and re-probed with anti-β-actin antibody. (D) Actin-normalized densitometry of TLR4 immunoblots.