Fig. 4

Evaluation of kidney injury. To evaluate kidney injury, BUN (a) and creatinine (b) were determined at the end of resuscitation. In addition, specimens of renal tissues were scored (c) after HE stain (e, 200×). Immunofluorescence stain of renal NGAL were performed to evaluate acute kidney injury (d, f). BUN (g) and creatinine (h) were also detectable in dialysis solutions collected from abdominal cavity. BUN = blood urea nitrogen; Con = control group; MFR = volume resuscitation; PPD = preventive peritoneal dialysis; NGAL = neutrophil gelatinase-associated lipocalin. In each group, number of animals ≥5 for biochemical analysis and number of animals ≥4 for morphological assessments. ^**p < 0.01, ^*** p < 0.001 vs. CP group; ^#p < 0.05, ^###p < 0.001 vs. MFR group; ^ΔΔ p < 0.01 vs. PPD group