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Table 2 Biomarkers and their analysers, analytical methods and quality criteria

From: Can we rely on out-of-hospital blood samples? A prospective interventional study on the pre-analytical stability of blood samples under prehospital emergency medicine conditions

Biomarkers in alphabetic order Analyser model Manufacturer Test method Frequency of quality controls (3 shifts per day.) Acceptable Root Mean Standard Deviation as defined by Rili-BAEK Range of interest as defined by Rili-BAEK Unit
Alpha-HBDH AU 680 Beckman Coulter Inc., Brea, United States Kinetic (decreasing) UV-Test at 37 °C Twice per 8 h-shift plus after every 50 specimen. not defined by Rili-BAEK not defined by Rili-BAEK U/l
Antithrombin III STA-R Evolution Diagnostica Stago S.A.S., Asnieres sur Seine, France Colorimetric Assay (ELISA) of Antithrombin III Activity 4 times per 8 h-shift. not defined by Rili-BAEK not defined by Rili-BAEK U/l
aPTT STA-R Evolution Diagnostica Stago S.A.S., Asnieres sur Seine, France Time to clotting after recalcification of plasma in the presence of cephalin (platelet substitute) and a factor XII activator (poly-phenolic component) 4 times per 8 h-shift. 10,5% 20–120 sec.
Calcium (ionized) ABL 800Flex Radiometer Medical ApS, Brønshøj, Denmark Potentiometric method Once per 8 h-shift. 14% 0,2–≤ 1 mmol/l
7,5% >1–2,5
Chloride ABL 800Flex Radiometer Medical ApS, Brønshøj, Denmark Potentiometric method Once per 8 h-shift. 4,5% 70–150 mmol/l
Creatin Kinase (CK) AU 680 Beckman Coulter Inc., Brea, United States International Federation of Clinical Chemistry and Laboratory Medicine Method Twice per 8 h-shift plus after every 50 specimen. 11% 50–1000 U/l
Creatinine AU 680 Beckman Coulter Inc., Brea, United States Kinetic colorimetric Jaffe reaction Twice per 8 h-shift plus after every 50 specimen. 11,5% 0,5–10 mg/dl
Fibrinogen STA-R Evolution Diagnostica Stago S.A.S., Asnieres sur Seine, France Clauss clotting assay 4 times per 8 h-shift. not defined by Rili-BAEK not defined by Rili-BAEK mg/dl
Free Haemoglobin Cobas Integra 400 Hoffmann- La Roche AG, Basel, Switzerland Colorimetric Test 4 times per 8 h-shift. 20% not defined by Rili-BAEK mg/l
Glutamic Oxaloacetic Transaminase (GOT/AST) AU 680 Beckman Coulter Inc., Brea, United States International Federation of Clinical Chemistry and Laboratory Medicine - Method Twice per 8 h-shift plus after every 50 specimen. 11,5% 20–400 U/l
Haemoglobin XE5000 Sysmex Corporation KK, Kobe, Japan SLS (sodium lauryl sulphate) haemoglobin method Once per 8 h-shift. 4% 2–20 g/dl
INR STA-R Evolution Diagnostica Stago S.A.S., Asnieres sur Seine, France Time to clotting after addition of Calcium-rich thromboplastine (Neoplastine CI plus) 4 times per 8 h-shift. not defined by Rili-BAEK not defined by Rili-BAEK n.n.
Leukocytes XE5000 Sysmex Corporation KK, Kobe, Japan Flow cytometry with dynamic focusing Once per 8 h-shift. 6,5% 2–30 109/l
Myoglobin AU 5800 Beckman Coulter Inc., Brea, United States electrochemiluminescence-immunoassay Twice per 8-h shift not defined by Rili-BAEK not defined by Rili-BAEK μg/l
Potassium ABL 800Flex Radiometer Medical ApS, Brønshøj, Denmark Potentiometric method Once per 8 h-shift. 4,5% 2–8 mmol/l
Sodium ABL 800Flex Radiometer Medical ApS, Brønshøj, Denmark Potentiometric method Once per 8 h-shift. 3% 110–180 mmol/l
Thrombocytes XE5000 Sysmex Corporation KK, Kobe, Japan Flow cytometry with dynamic focusing; traditional impedance technology for very low and very high PLT counts Once per 8 h-shift. 13,5% 40–≤150 109/l
8,5% 150–≤300
7,5% 300–700
Troponin I Access 2 Beckman Coulter Inc., Brea, United States electrochemiluminescence immunoassay 4 times per 8 h-shift. 20% 0,1–35 μg/l
  1. For more detailed information on the laboratory testing of our selected parameters we refer to the technical manuals and reference guidebooks of each analyser as well as the Guideline of the German Medical Association on Quality Assurance in Medical Laboratory Examinations (Rili-BAEK [15])